一篇不是很新的文章,本文思路是从转录水平研究基因表达调控。分别从反式作用因子之间,和反式和顺式的相互作用过程。
TARDBP蛋白是肿瘤启动子(反式作用因子)借助非编码RNA MiR500a-3p(反式作用因子)会抑制靶基因LIF和PAPPA(顺式作用元件)的表达; TARDBP(反式作用因子)与成熟miRNAs结合 ; 同时, TARDBP(反式作用因子)调控miRNAs(反式作用因子)的异构体和臂的选择。
研究步骤:通过体内、体外实验确定直接调控关系;然后在分子层面上阐明相互作用的顺式元件(靶向基因)在启动子(MiRNA和TADBP)上所处的位置(miR-500a-3p与LIF和PAPPA mRNA的3'UTR区域相互作用)。
最后,有些文章还会分析反式作用因子(miRNA)及其顺式作用元件相互作用的动力学特征,但是本文没有。相关研究手段有ChIP-chip/seq、genomic SELEX、细菌单杂交技术等, 凝胶阻滞实验 (Electrophoretic mobility shift assay,EMSA)。#基因表达调控##TARDBP#
TARDBP蛋白是肿瘤启动子(反式作用因子)借助非编码RNA MiR500a-3p(反式作用因子)会抑制靶基因LIF和PAPPA(顺式作用元件)的表达; TARDBP(反式作用因子)与成熟miRNAs结合 ; 同时, TARDBP(反式作用因子)调控miRNAs(反式作用因子)的异构体和臂的选择。
研究步骤:通过体内、体外实验确定直接调控关系;然后在分子层面上阐明相互作用的顺式元件(靶向基因)在启动子(MiRNA和TADBP)上所处的位置(miR-500a-3p与LIF和PAPPA mRNA的3'UTR区域相互作用)。
最后,有些文章还会分析反式作用因子(miRNA)及其顺式作用元件相互作用的动力学特征,但是本文没有。相关研究手段有ChIP-chip/seq、genomic SELEX、细菌单杂交技术等, 凝胶阻滞实验 (Electrophoretic mobility shift assay,EMSA)。#基因表达调控##TARDBP#
人就这么一辈子,生不带来死不带去的一辈子。我们在亲人的欢笑声中诞生,又在亲人的悲伤中离去。而这一切我们都不知道,我们无法控制自己的生与死,但我们应庆幸自己拥有了这一辈子。一个人惟有敢于正视现实,正视错误,用理智分析,彻底感悟,才不至于被回忆侵蚀。
People are so a lifetime, life does not bring death does not bring a lifetime. We were born in the laughter of loved ones, and left in the sadness of loved ones. We can't control our own life and death, but we should be glad that we have this life. Only a person can dare to face up to reality, face up to mistakes, rational analysis, thorough perception, will not be eroded by memories.
People are so a lifetime, life does not bring death does not bring a lifetime. We were born in the laughter of loved ones, and left in the sadness of loved ones. We can't control our own life and death, but we should be glad that we have this life. Only a person can dare to face up to reality, face up to mistakes, rational analysis, thorough perception, will not be eroded by memories.
#左航ZH[超话]##左航 绝对舞台掌控者##左航超话发帖量破900w第一人#
你平静地做好自己的事,生活自会把属于你的东西,在适合的时候,一样一样都给你,好的人生,不慌不忙。
If you do your own things calmly, life will give you all the things that belong to you at the right time. A good life is not in a hurry.
你平静地做好自己的事,生活自会把属于你的东西,在适合的时候,一样一样都给你,好的人生,不慌不忙。
If you do your own things calmly, life will give you all the things that belong to you at the right time. A good life is not in a hurry.
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